Plasma glutathione reductase activity and prognosis of septic shock

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• 作者：Jae Seong Kim ; MD^a ; Woon Yong Kwon ; MD ; PhD^a ; ^{kwy711@hanmail.net" class="auth_mail" title="E-mail the corresponding author} ; Gil Joon Suh ; MD ; PhD^a ; Kyung Su Kim ; MD ; PhD^a ; Yoon Sun Jung ; MD^b ; Sung Hee Kim ; MSc^a ; So Eun Lee ; MD^c
• 关键词：Septic shock ; Oxidative stress ; Glutathione ; Oxidation reduction ; Glutathione reductase ; Mortality
• 刊名：Journal of Surgical Research
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：200
• 期：1
• 页码：298-307
• 全文大小：735 K

文摘

Our aim was to investigate whether plasma glutathione reductase (GR) activity is well correlated with the erythrocyte-reduced glutathione (GSH)/glutathione disulfide (GSSG) ratio and is associated with the mortality of septic shock.

Materials and methods

This study was conducted on male Sprague–Dawley rats and patients admitted to the intensive care unit with septic shock. To induce endotoxemia in rats, vehicle or lipopolysaccharide (LPS) at dosages of 5 or 10 mg/kg were injected into a tail vein. Animals were then euthanized 6 h post-LPS. Based on the 28-d mortality, the enrolled patients were divided into the survivors and nonsurvivors. We obtained blood samples from patients at admission (0 h) and 24 h after admission to the intensive care unit.

Results

In endotoxemic rats, the erythrocyte GSH/GSSG ratio, erythrocyte GR activity, and plasma GR activity in the 10 mg/kg of LPS group were lower than those in the sham and 5 mg/kg of LPS groups. In patients with septic shock, decrease in plasma GR activity at 24 h was independently associated with an increase in 28-d mortality (odds ratio, 0.828; 95% confidence interval, 0.690–0.992, P = 0.041). Plasma GR activity was correlated with erythrocyte GR activity (Spearman ρ = 0.549, P < 0.001) and the erythrocyte GSH/GSSG ratio (rho = 0.367, P = 0.009) at 24 h.

Conclusions

Plasma GR activity was well correlated with erythrocyte GR activity and the erythrocyte GSH/GSSG ratio, and a decrease in plasma GR activity was associated with an increase in the mortality of septic shock patients.