Analysis of cag-8: A novel poly(Q)-encoding gene in the mouse brain
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- 作者：Jin Woo Ji ; Hye Lim Yang and Sun Jung Kim
- 关键词：Mouse brain ; Poly(Q) ; Trinucleotide repeat ; Alternative splicing ; 3′ ; -Untranslated region
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2006
- 出版时间：11 August 2006
- 年：2006
- 卷：346
- 期：4
- 页码：1254-1260
- 全文大小：1466 K
文摘
We identified 13 transcript isoforms of a trinucleotide-repeat-containing gene, cag-8, that is expressed almost exclusively in the mouse brain. The polypeptide deduced from the cDNA consists of 137 AAs, of which 74 are glutamines. The 130-kb gene is composed of 16 exons, from which at least 13 isoforms with variable UTRs are formed by alternative splicing. A strong positive cis-acting element was found in the neuroblastoma × glioma hybrid NG108-15 and human embryonic kidney HEK293 cell lines. The cag-8 protein was localized in the cytosol and in granular bodies within the nucleus. These findings indicate that cag-8 is a novel poly(Q)-encoding gene, the expression of which is confined primarily to the brain.