Resonance light scattering technique as a new tool to determine the binding mode of anticancer drug oridonin to?DNA

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• 作者：Zhanguang Chen ; Zhen Wang ; Junhui Chen ; Xi Chen ; Jiahui Wu ; Yanyu Wu ; Jieyao Liang
• 关键词：Oridonin ; Resonance light scattering ; DNA ; Intracellular target ; Binding mode
• 刊名：European Journal of Medicinal Chemistry
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：66
• 期：Complete
• 页码：380-387
• 全文大小：1872 K

文摘

In this study, a sensitive and reliable assay has been developed to determine the binding mode of oridonin to DNA in?vitro using resonance light scattering (RLS) technique. The binding mode of ORI with DNA has been confirmed by measuring the changes of RLS intensity under different pH and ionic strength. It is the ORI that is intercalated into the double helix DNA to destroy its template function and inhibit the synthesis of DNA. Moreover, the RLS assay result was validated by seasoned vitro methods. The proposed RLS assay is not only an unprecedented one for primarily determine the binding mode of oridonin to DNA, but also a simple, sensitive, objective and straightforward method for the detection of?ORI.