Enhancement of methanol resistance of Yarrowia lipolytica lipase 2 using β-cyclodextrin as an additive: Insights from experiments and molecular dynamics simulation
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- 作者：Hao Caoa ; 1 ; Yang Jianga ; 1 ; Haiyang Zhanga ; Kaili Niea ; b ; Ming Leic ; Li Denga ; b ; dengli@mail.buct.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Fang Wanga ; Tianwei Tana ; twtan@mail.buct.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：β-cyclodextrin ; Lipase ; Methanol resistance ; Surface modification ; Molecular dynamics
- 刊名：Enzyme and Microbial Technology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：96
- 期：Complete
- 页码：157-162
- 全文大小：1880 K
文摘
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Methanol resistance of lipase is increased by utilizing β-cyclodextrin.
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Experimental and computational results correspond well.
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Mechanism of methanol resistance of lipase with β-cyclodextrin is revealed.
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Conformation of lipase in methanol is stabilized by β-cyclodextrin.
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β-cyclodextrin and lipase interact through hydrogen bond.