Micro-computed tomography and microdissection of the temporal bone of tree shrews

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• 作者：Xie Lihong¹ ; Li Heng¹ ; Bibek Gyanwali ; Zhu Meichan ; Zhu Kaiquan ; Sun Kai ; Tang Anzhou ; ^{anzhoutang@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Tree shrews ; Micro-CT ; Temporal bone ; Three-dimensional reconstruction ; Anatomy
• 刊名：Annals of Anatomy - Anatomischer Anzeiger
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：208
• 期：Complete
• 页码：69-77
• 全文大小：1675 K

文摘

To understand the morphology and anatomical data of the temporal bone of tree shrews through micro-computed tomography (micro-CT) and microdissection.

Methods

Skull specimens from 10 tree shrews were scanned using micro-CT examination. The acquired images were used for three-dimensional reconstruction and measurement using the Mimics 10.01 software. Twenty tree shrews were subjected to microdissection and the data were measured.

Results

Micro-CT and three-dimensional reconstruction could clearly define the three-dimensional spatial position of the ear structure. Micro-CT and microdissection showed that the otic vesicles of the tree shrews were located on both sides of posterior-inferior skull bone. The location of the otic vesicles was superficial, and the bone was thin. All of the structures of the middle and inner ear of the tree shrews were well developed. The ossicular chain was differentiated into the malleus, incus and stapes. The location of the three semi-circular canals of the tree shrews was superficial and easy to dissect. In vivo, the three semi-circular canals were easy to localize and the surface bone was thin. The contour and structure of the cochlea and number of cochlear turns were similar to those in humans.

Conclusion

This study could provide anatomical data to allow tree shrews to be used as animal models for studying ear diseases.