Tracking protein-protein interaction and localization in living cells using a high-affinity molecular binder
详细信息 查看全文
- 作者：Hee-Yeon Kima ; Joong-jae Leeb ; Nury Kimb ; Won Do Heob ; ; Hak-Sung Kimb ; hskim76@kaist.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Protein&ndash ; protein interactions ; Red fluorescent protein ; Intracellular antibody ; Colocalization analysis ; Live-cell imaging
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：19 February 2016
- 年：2016
- 卷：470
- 期：4
- 页码：857-863
- 全文大小：1751 K
文摘
- •
We demonstrated that a repebody can be used for tracking protein–protein interaction through live-cell imaging.
- •
A mCherry-specific repebody was used for tracing a target protein in mammalian cells.
- •
The present approach can be generally applied for diverse target proteins in living cells.