Comparisons of the effects of the sevoflurane and propofol on acute ischemia reperfusion and DNA damages in rabbits
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- 作者：Sema Oncul ; serdenish@yahoo.com ; Lale Karabiyik ; Erdem Coskun ; Ela Kadioglu ; Ozlem Gulbahar
- 关键词：Genotoxicity ; Oxidative stress ; Propofol ; Sevoflurane ; Ischemia reperfusion injury
- 刊名：Brazilian Journal of Anesthesiology (English Edition)
- 出版年：2017
- 出版时间：January-February 2017
- 年：2017
- 卷：67
- 期：1
- 页码：35-41
- 全文大小：762 K
- 卷排序：67
文摘
The aim of this study was to compare the effects of sevoflurane and propofol anesthesia on oxidative DNA damage that occurs in low-extremity ischemia and is caused by tourniquet application.MethodsFourteen New Zealand rabbits were randomly allocated into two equal groups. Group S (n = 7) received sevoflurane (2.5–4 percent) inhalation and Group P (n = 7) received a propofol infusion (1–2 mg·kg−1·min−1), after which a pneumatic tourniquet was placed on the right lower extremity. Blood samples were collected prior to tourniquet placement (baseline), 120 min after ischemia, 15 min after ischemia and 120 minutes (min) after ischemia. Malondialdehyde (MDA) levels were analyzed to determine lipid peroxidation, and single cell gel electrophoresis (SCGE) was used to determine DNA damage.ResultsAt 15 min after ischemia, the MDA levels in Group P (8.15 ± 2.61 μM) were higher than baseline (6.26 ± 3.19 μM, p = 0.026) and Group S (4.98 ± 0.77 μM, p = 0.01). DNA damage was similar in both groups, although DNA damage was higher than baseline (tail moment 0.63 ± 0.27, tail intensity 3.76 ± 1.26) in Group P at the 15th minute of reperfusion (tail moment 1.05 ± 0.45, p = 0.06; tail intensity 5.33 ± 1.56, p = 0.01). The increase in tail moment and tail intensity returned to normal levels in both groups 2 hours after the termination of ischemia.ConclusionGiven that oxidative stress and genotoxic effect disappear in the late stages of reperfusion, we conclude that neither sevoflurane nor propofol can be considered superior to the other in anesthesia practices for extremity surgeries involving the use of a tourniquet.