Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes Application in fluoroimmunoassay
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- 作者：Laukkanen ; Marja-Leena ; Orellana ; Adelina ; Keinä ; nen ; Kari
- 关键词：Antibody engineering ; Europium chelate ; Immunoliposome ; Lanthanide ; Lipid-tagged antibody ; Time-resolved fluorometry
- 刊名：Journal of Immunological Methods
- 出版年：1995
- 出版时间：September 11, 1995
- 年：1995
- 卷：185
- 期：1
- 页码：95-102
- 全文大小：609 K
文摘
Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes. The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay. These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.