Rap2 function requires palmitoylation and recycling endosome localization

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• 作者：Yukiko Uechi ; Maitsetseg Bayarjargal ; Masato Umikawa ; Minoru Oshiro ; Kimiko Takei ; Yoshito Yamashiro ; Tsuyoshi Asato ; Shogo Endo ; Ryo Misaki ; Tomohiko Taguchi ; Ken-ichi Kariya
• 关键词：Rap2 ; Ras ; Small G protein ; Post-translational processing ; TNIK ; Mitogen-activated protein kinase kinase kinase kinase ; Recycling endosomes
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2009
• 出版时间：23 January 2009
• 年：2009
• 卷：378
• 期：4
• 页码：732-737
• 全文大小：360 K

文摘

Rap2A, Rap2B, and Rap2C are Ras-like small G proteins. The role of their post-translational processing has not been investigated due to the lack of information on their downstream signaling. We have recently identified the Traf2- and Nck-interacting kinase (TNIK), a member of the STE20 group of mitogen-activated protein kinase kinase kinase kinases, as a specific Rap2 effector. Here we report that, in HEK293T cells, Rap2A (farnesylated) and Rap2C (likely farnesylated), but not Rap2B (geranylgeranylated), require palmitoylation for membrane-association and TNIK activation, whereas all Rap2 proteins, including Rap2B, require palmitoylation for induction of TNIK-mediated phenotype, the suppression of cell spreading. Furthermore, we report for the first time that, in COS-1 cells, Rap2 proteins localize, and recruit TNIK, to the recycling endosomes, but not the Golgi nor the endoplasmic reticulum, in a palmitoylation-dependent manner. These observations implicate the involvement of palmitoylation and recycling endosome localization in cellular functions of Rap2 proteins.