Identification of K^k-restricted T-cell epitope within influenza virus nucleoprotein

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• 作者：Kuroda ; Kazumichi ; Nakajima ; Mariko ; Minakata ; Hiroyuki ; Hosaka ; Yasuhiro
• 关键词：Influenza virus ; Cytotoxic T-lymphocyte ; T-cell epitope
• 刊名：Virus Research
• 出版年：1995
• 出版时间：September, 1995
• 年：1995
• 卷：38
• 期：1
• 页码：43-53
• 全文大小：546 K

文摘

In order to determine the epitope structure in peptide NP50-63, which has been reported to be the only K^k-restricted T-cell antigen within the influenza virus (A/PR/8/34) nucleoprotein, a series of 13 peptides truncated from C- and N-termini of NP50-63 were synthesized and their sensitizing activities against K^k-restricted nucleoprotein-specific cytotoxic T lymphocytes (CTLs) were examined. One of the 13 peptides, NP50-57, sensitized L929 cells at the nM level, which was 100-1000 times lower in concentration than that at which the other peptides sensitized these cells. The presence of NP50-57 in A/PR/8/34-infected L929 cells was also investigated. Acid extracts of virus-infected cells were separated on a reverse-phase HPLC column and then anion-exchange column. By both separations, only one peak of sensitizing activity against nucleoprotein-specific CTLs was observed. The position of the peak coincided with that of the elution of NP50-57. These results strongly suggest that NP50-57 is the natural epitope in the antigenic structure, NP50-63.