Quercetin-3-O-glucuronide induces ABCA1 expression by LXR伪 activation in murine macrophages

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• 作者：Kazuaki Ohara ; Hideyuki Wakabayashi ; Yoshimasa Taniguchi ; Kazutoshi Shindo ; Hiroaki Yajima ; Aruto Yoshida
• 关键词：ABCA1 ; ATP-binding cassette ; subfamily A ; member 1 ; HDL ; high density lipoprotein ; HDLC ; high density lipoprotein-cholesterol ; LXR伪 ; liver X receptor alpha ; NNE ; Nelumbo nucifera leaf extracts ; Q3GA ; quercetin-3-O-glucuronide ; RCT ; reverse cholesterol transport
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：29 November, 2013
• 年：2013
• 卷：441
• 期：4
• 页码：929-934
• 全文大小：513 K

文摘

Reverse cholesterol transport (RCT) removes excess cholesterol from macrophages to prevent atherosclerosis. ATP-binding cassette, subfamily A, member 1 (ABCA1) is a crucial cholesterol transporter involved in RCT to produce high density lipoprotein-cholesterol (HDLC), and is transcriptionally regulated by liver X receptor alpha (LXR伪), a nuclear receptor. Quercetin is a widely distributed flavonoid in edible plants which prevented atherosclerosis in an animal model. We found that quercetin-3-O-glucuronide (Q3GA), a major quercetin metabolite after absorption from the digestive tract, enhanced ABCA1 expression, in vitro, via LXR伪 in macrophages. In addition, leaf extracts of a traditional Asian edible plant, Nelumbo nucifera (NNE), which contained abundant amounts of quercetin glycosides, significantly elevated plasma HDLC in mice. We are the first to present experimental evidence that Q3GA induced ABCA1 in macrophages, and to provide an alternative explanation to previous studies on arteriosclerosis prevention by quercetin.