Interaction of plectin and intermediate filaments

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• 作者：Tadashi Karashima ; Daisuke Tsuruta ; Takahiro Hamada ; Norito Ishii ; Fumitake Ono ; Keiko Hashikawa ; Bungo Ohyama ; Yohei Natsuaki ; Shunpei Fukuda ; Hiroshi Koga ; Ryosuke Sogame ; Takekuni Nakama ; Teruki Dainichi ; Takashi Hashimoto ; ^{hashimot@med.kurume-u.ac.jp}
• 关键词：Plakin ; Vimentin ; Keratin ; Plectin ; Intermediate filament
• 刊名：Journal of Dermatological Science
• 出版年：2012
• 出版时间：April, 2012
• 年：2012
• 卷：66
• 期：1
• 页码：44-50
• 全文大小：2002 K

文摘

Background

Plectin, a member of the plakin family proteins, is a high molecular weight protein that is ubiquitously expressed. It acts as a cytolinker for the three major components of the cyotoskeleton, namely actin microfilaments, microtubules and intermediate filaments.

Objective

The aim of our experiments was to identify new binding sites for intermediate filaments on plectin and to specify these sites.

Methods

We introduced truncated forms of plectin into several cell lines and observe interaction between plectin and intermediate filaments.

Results

We found that a linker region in the COOH-terminal end of plectin was required for the association of the protein with intermediate filaments. In addition, we also demonstrated that a serine residue at position 4645 of plectin may have a role on binding of plectin to intermediate filaments.

Conclusion

A linker region in the COOH-terminal end and serine residue at position 4645 may be important for the binding of plectin to intermediate filaments.