P-Selectin Expression in Cold Preserved Kidneys in University of Wisconsin and Histidine-Tryptophan-Ketoglutarate Solutions

详细信息    查看全文

• 作者：Dawit Worku M.D. ; M.Sc.^* ; ^{dworku@tulane.edu"" rel=""nofollow} ; Sebastian Laluf M.D.^* ; Jennifer McGee M.D.^* ; Monica Goswami M.D.^* ; Keith VanMeter M.D.^† ; ; Douglas P. Slakey M.D. ; M.P.H.^*
• 关键词：University of Wisconsin ; histidine-tryptophan-ketoglutarate ; P-selectin ; kidney transplantation
• 刊名：Journal of Surgical Research
• 出版年：2011
• 出版时间：July 2011
• 年：2011
• 卷：169
• 期：1
• 页码：125-131
• 全文大小：1134 K

文摘

The differences and efficacy of standard preservation solutions in kidney transplantation, University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK), remain a topic of debate in recent clinical studies. P-selectins represent glycoproteins expressed on endothelial cells and platelets responsible for the earliest events in ischemia/reperfusion injury in kidney transplantation. This study aimed to compare the levels of P-selectin expression between cold preserved kidney tissues in UW and HTK solutions. Thirty kidneys were procured from male Lewis rats and stored in cold (4°C) solutions for periods of 4, 12, 16, 20, and 24h. Group 1 (n = 15) kidneys were stored in UW solutions, and group 2 (n = 15) kidneys were submerged in HTK solutions. At the end of each time point, the kidneys underwent preparation and levels of P-selectin expression in the tissues were measured using Immunoblot analyses and adjusted volumetric quantification of Western blot signals. For all periods of cold preservation, P-selectin expression was significantly down-regulated in kidney tissues stored in UW compared with HTK solutions (P < 0.001). In summary, UW demonstrated a significant benefit over HTK solution in down-regulating P-selectin expression in cold preserved kidney grafts.