Characteristics of human cell line, F2N78, for the production of recombinant antibody in fed-batch and perfusion cultures

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• 作者：Joon Serk Seo¹ ; Byung Sub Min² ; Young-Bum Kwon² ; Soo-Young Lee² ; Jong-Moon Cho² ; Keun-Hee Park² ; Yae Ji Yang² ; Ki Eun Maeng² ; Shin-Jae Chang² ; Dong-Il Kim¹ ; ^{kimdi@inha.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Human cells ; F2N78 cells ; Fed-batch culture ; Perfusion culture ; Glycosylation
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：121
• 期：3
• 页码：317-324
• 全文大小：1289 K

文摘

A human hybrid cell line, F2N78, was developed by somatic fusion of HEK293 and Namalwa cells for the production recombinant biopharmaceutical proteins. In this study, we performed perfusion culture to verify its potential in culture process used for human cell expression platform. Cell viability could be maintained over 90% and high viable cell density was obtained at higher than 1.0 × 10⁷ cells/mL by bleeding process in perfusion culture. The cells were adapted well in both culture modes, but there were apparent differences in protein quality. Compared to fed-batch culture, degalactosylated forms such as G0F and G0 as well as Man5 showed no significant increases in perfusion culture. In terms of charge variants, acidic peaks increased, whereas main peaks constantly decreased according to the length of culture period in both methods.