Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems

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• 作者：Yu Kiat Lin ; Chien Wei Ooi ; Joo Shun Tan ; Pau Loke Show ; Arbakariya Ariff ; Tau Chuan Ling
• 关键词：Aqueous two-phase system ; Interferon-alpha2b ; Alcohol/salt system ; Purification
• 刊名：Separation and Purification Technology
• 出版年：13 December, 2013
• 年：2013
• 卷：120
• 期：Complete
• 页码：362-366
• 全文大小：501 K

文摘

The purification of intracellular human recombinant interferon-alpha2b (IFN-伪2b) from Escherichia coli (E. coli) was studied using alcohol/salt aqueous two-phase system (ATPS). The influences of nine biphasic systems comprising alcohol-based top phase (ethanol, 1-propanol and 2-propanol) and salt-based bottom phase (ammonium sulfate, di-potassium hydrogen phosphate and monosodium citrate) on IFN-伪2b purification were studied. The results showed that the optimum condition for purification of IFN-伪2b was achieved in ATPS composed of 18% (w/w) 2-propanol with 22% (w/w) ammonium sulfate in the presence of 1% (w/w) sodium chloride (NaCl). The purified IFN-伪2b recorded a purification factor (P<sub>Fsub>) of 16.24 with the yield of 74.64%.