Gambogic acid induces apoptotic cell death in T98G glioma cells

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• 作者：Mya Thida^a ; ^&dagger ; ; Dae Won Kim^b ; ^&dagger ; ; Thi Thu Thuy Tran^c ; Minh Quan Pham^c ; Heesu Lee^d ; Inki Kim^e ; ^f ; ^{ik.kim@amc.seoul.kr" class="auth_mail" title="E-mail the corresponding author} ; Jae Wook Lee^a ; ^g ; ^{jwlee5@kist.re.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Gambogic acid ; Reactive oxygen species (ROS) ; Glioblastoma ; Apoptosis ; Anticancer
• 刊名：Bioorganic & Medicinal Chemistry Letters
• 出版年：2016
• 出版时间：1 February 2016
• 年：2016
• 卷：26
• 期：3
• 页码：1097-1101
• 全文大小：1101 K

文摘

Gambogic acid (GA), a natural product with a xanthone structure, has a broad range of anti-proliferative effects on cancer cell lines. We evaluated GA for its cytotoxic effects on T98G glioblastoma cells. GA exhibited potent anti-proliferative activity and induced apoptosis in T98G glioblastoma cells in a dose-dependent manner. Incubation of cells with GA revealed apoptotic features including increased Bax and AIF expression, cytochrome c release, and cleavage of caspase-3, -8, -9, and PARP, while Bcl-2 expression was downregulated. Furthermore, GA induced reactive oxygen species (ROS) generation in T98G cells. Our results indicate that GA increases Bax- and AIF-associated apoptotic signaling in glioblastoma cells.