A graphene oxide-based multiplexed fluorescence assay for the detection of protein kinase activity in cell lysates and the evaluation of protein kinase inhibition
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- 作者：Yong Huang ; huangyong_2009@163.com" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Huakui Huang ; Jian Qin ; Xiaoqian Liu ; Shulin Zhao ; zhaoshulin001@163.com" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Zhen-Feng Chen ; Hong Liang ; hliang@gxnu.edu.cn" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：Graphene oxide ; Multiplexed detection ; Protein kinase ; Cell lysates
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：238
- 期：Complete
- 页码：908-916
- 全文大小：2220 K
文摘
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A novel graphene oxide sensing platform for multiplexed detection of protein kinases was developed.
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The proposed sensing platform exhibits high sensitivity and specificity.
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This platform was applied for quantification of multiple protein kinases in cell lysates and screening kinase inhibitors.
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This approach may provide a routine tool for the high-throughput screening of protein kinases and their inhibitors.