Determination of human serum 8-hydroxy-2′-deoxyguanosine (8-OHdG) by HPLC-ECD combined with solid phase extraction (SPE)
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- 作者：Satoshi Koide ; Yuri Kinoshita ; Narushi Ito ; Jun Kimura ; Kenji Yokoyama ; Isao Karube
- 关键词：Human serum ; 8-Hydroxy-2′ ; – ; deoxyguanosine (8-OHdG) ; Solid phase extraction (SPE) ; High performance liquid chromatography (HPLC) ; Electrochemical detector (ECD)
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2010
- 出版时间：1 August 2010
- 年：2010
- 卷：878
- 期：23
- 页码：2163-2167
- 全文大小：231 K
文摘
Human serum 8-hydroxy-2′-deoxyguanosine (8-OHdG) was measured by HPLC-ECD method combined with solid phase extraction (SPE) developed by our group: (our proprietary kit, named 8-OHdG Pre-treatment Kit (TANITA Corporation)). The major interfering substances and proteins in serum were removed by 8-OHdG Pre-treatment Kit. This measurement method was highly reproducible (CV = 2.2–7.1 % ) and demonstrated the lower detection limit for control serum sample of less than 10 pg/ml without the sample evaporation. The other hand 8-OHdG concentration in serum for healthy people was in the range of 0–70 pg/ml (25.5 ± 13.8 pg/ml, n = 37). Secondary a relationship between the HPLC-ECD and ELISA methods was investigated. ELISA method could not detect 8-OHdG concentration in serum for healthy people, because the detection limit of 130 pg/ml was higher than the normal range for healthy people. These results show our SPE method has high sensitivity and quantitative accuracy for 8-OHdG analysis.