n-3 Fatty acids regulate the inflammatory-state related genes in the lung epithelial cells exposed to polycyclic aromatic hydrocarbons

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• 作者：Joanna Gdula-Argasińska^a ; ^{jargasinska@cm-uj.krakow.pl" class="auth_mail" title="E-mail the corresponding author} ; Jacek Czepiel^b ; Justyna Totoń-Żurańska^c ; ^d ; Paweł Wołkow^c ; ^d ; Tadeusz Librowski^a ; Anna Czapkiewicz^f ; William Perucki^g ; Michał Woźniakiewicz^e ; Aneta Woźniakiewicz^e
• 关键词：AA ; arachidonic acid ; AHR ; aryl hydrocarbon receptor ; ARNT ; aryl hydrocarbon nuclear translocator ; BaP ; benzo(a)pyrene ; Baa ; benzo(a)anthracene ; Chr ; chrysene cyclooxygenase-2 ; CYP1 A1 ; cytochrome P450 1 A1 ; DHA ; docosahexaenoic acid ; EPA ; eicosapentaenoic acid ; FAs ; fatty acids ; Flu ; fluoranthene ; MUFA ; monounsaturated fatty acids ; NF-κB ; nuclear transcription factor κB ; PAHs ; polycyclic aromatic hydrocarbons ; PGF2α ; prostaglandin F2α ; PGF3α ; prostaglandin F3α ; PLA2G4A ; phospholipase A2 IV (cPL
• 刊名：Pharmacological Reports
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：68
• 期：2
• 页码：319-328
• 全文大小：1752 K

文摘

Chronic airway inflammation is coordinated by a complex of inflammatory mediators, including eicosanoids. The aim of this study was to evaluate the impact of polycyclic aromatic hydrocarbons (PAHs) on the human lung epithelial carcinoma A549 cells supplemented with docosahexaenoic (DHA) and eicosapentaenoic (EPA) acids.

Methods

We analyzed the influence of DHA, EPA and/or benzo(a)pyrene (BaP), chrysene (Chr), fluoranthene (Flu) and benzo(a)anthracene (Baa) treatment on the fatty acids (FAs) profile and the formation of isoprostanes. We studied the cyclooxygenase-2, FP-receptor, peroxisome proliferator-activated receptors PPARδ and PPARγ, transcription factor NF-кB p50 and p65 expression by Western blot, phospholipase A2 (cPLA2) activity, as well as aryl hydrocarbon receptor (AHR), cytochrome P450 (CYP1A1), phospholipase A2 (PLA2G4A) and prostaglandin synthase 2 (PTGS2) gene expression by qRT-PCR.

Results

DHA or EPA supplementation and BaP or Baa treatment resulted in a higher level of PGF3α. COX-2 expression was decreased while PPARδ expression and cPLA2 activity was increased after fatty acid supplementation and PAHs treatment. DHA and EPA up-regulated AHR and PLA2G4A genes.

Conclusions

Supplementation with n-3 FAs resulted in changes of inflammatory-state related genes in the lung epithelial cells exposed to PAHs. The altered profile of lipid mediators from n-3 FA as well as repression of the COX-2 protein by n-3 PUFAs in A549 cells incubated with PAHs suggests anti-inflammatory and pro-resolving properties of DHA and EPA. It remains to be shown whether these pleiotropic and protective actions of n-3 FAs contribute to fish oil's therapeutic effect in asthma.