The fungi Trichoderma spp., Aspergillus niger, Penicillium italicum and Fusarium spp. have been developed on the halophyte plant, Juncus maritimes, producing polysaccharides hydrolases activities, i.e. endoglucanasecatalyses cellulose hydrolysis, and beta-glucosidasecatalyses hydrolysis of terminal non-reducing residues in beta-glucosides. The saccharification was carried out with an enzymatic preparation extracted from filamentous fungus Trichoderma spp. Experimental Doehlert design was performed to optimize the reducing sugars concentration. High sugar yields (8.5聽g/L) were obtained using a small amount of the extracted enzyme without stirring.
The highest enzymatic activities of endoglucanase and beta-glucosidases were produced by Trichoderma spp. at 22.12 and 0.07聽U/mg, respectively.