Effect of lactate and pH on mouse pluripotent stem cells: Importance of media analysis

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• 作者：Priyanka Gupta^a ; ^b ; ^c ; ¹ ; Kerry Hourigan^b ; ^d ; Sameer Jadhav^c ; Jayesh Bellare^c ; Paul Verma^b ; ^e ; ^{paul.verma@sa.gov.au}
• 关键词：Lactate ; Mouse pluripotent stem cell ; pH ; 2D expansion
• 刊名：Biochemical Engineering Journal
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：118
• 期：Complete
• 页码：25-33
• 全文大小：2495 K
• 卷排序：118

文摘

Medium pH and exogenous lactate addition are found to be important for mPSC culture. Change in extracellular media pH affects stem cell growth as well as pluripotency. External addition of lactate to culture media affects cell growth, not pluripotency. Lactate concentration in medium decreases on addition of exogenous sodium lactate. Exogenous lactate either quenches lactate production or is utilized by cells.