Efficient enzymatic synthesis of l-rhamnulose and l-fuculose

详细信息    查看全文

• 作者：Liuqing Wen^a ; ^&dagger ; ; Lanlan Zang^b ; ^&dagger ; ; Kenneth Huang^a ; Shanshan Li^a ; Runling Wang^b ; ^{wangrunling@tmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Peng George Wang^a ; ^{pwang11@gsu.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：l-Rhamnulose ; l-Fuculose ; Enzymatic synthesis ; Phosphorylation ; De-phosphorylation
• 刊名：Bioorganic & Medicinal Chemistry Letters
• 出版年：2016
• 出版时间：1 February 2016
• 年：2016
• 卷：26
• 期：3
• 页码：969-972
• 全文大小：802 K

文摘

l-Rhamnulose (6-deoxy-l-arabino-2-hexulose) and l-fuculose (6-deoxy-l-lyxo-2-hexulose) were prepared from l-rhamnose and l-fucose by a two-step strategy. In the first reaction step, isomerization of l-rhamnose to l-rhamnulose, or l-fucose to l-fuculose was combined with a targeted phosphorylation reaction catalyzed by l-rhamnulose kinase (RhaB). The by-products (ATP and ADP) were selectively removed by silver nitrate precipitation method. In the second step, the phosphate group was hydrolyzed to produce l-rhamnulose or l-fuculose with purity exceeding 99% in more than 80% yield (gram scale).