Circumventing substrate interference in the Raman spectroscopic identification of blood stains

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• 作者：Gregory McLaughlin ; Vitali Sikirzhytski ; Igor K. Lednev
• 关键词：Raman spectroscopy ; Blood detection ; Substrate interference ; Forensic science ; Statistical analysis
• 刊名：Forensic Science International
• 出版年：2013
• 出版时间：10 September, 2013
• 年：2013
• 卷：231
• 期：1-3
• 页码：157-166
• 全文大小：1709 K

文摘

Raman spectroscopy has demonstrated remarkable capabilities in identifying blood in controlled laboratory conditions. However, substrate interference presents a significant challenge toward characterizing body fluid traces with Raman spectroscopy at a crime scene. Here, several possible solutions are explored, including the selection of laser excitation, isolating the signal of blood using spectral subtraction and using a favorable substrate for collection which minimizes interference. Simulated blood stain evidence was prepared and analyzed using a Raman microscope with variable laser capabilities. It is shown that the best approach for detecting blood depends on the nature of the substrate and the type of interference encountered.