Levels of supramolecular chirality of polyglutamine aggregates revealed by vibrational circular dichroism
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- 作者：Dmitry Kurouski ; Karunakar Kar ; Ronald Wetzel ; Rina K. Dukor ; Igor K. Lednev ; Laurence A. Nafie
- 关键词：PolyQ ; polyglutamine ; VCD ; vibrational circular dichroism ; DUVRR ; deep UV resonance Raman ; IR ; infrared ; AFM ; atomic force microscopy ; EM ; electron microscopy ; H/D ; hydrogen-deuterium
- 刊名：FEBS Letters
- 出版年：2013
- 出版时间：5 June, 2013
- 年：2013
- 卷：587
- 期：11
- 页码：1638-1643
- 全文大小：537 K
文摘
Polyglutamine (PolyQ) aggregates are a hallmark of several severe neurodegenerative diseases, expanded CAG-repeat diseases in which inheritance of an expanded polyQ sequence above a pathological threshold is associated with a high risk of disease. Application of vibrational circular dichroism (VCD) reveals that these PolyQ fibril aggregates exhibit a chiral supramolecular organization that is distinct from the supramolecular organization of previously observed amyloid fibrils. PolyQ fibrils grown from monomers with Q repeats 35 and above (Q ? 35) exhibit approximately 10-fold enhancement of the same VCD spectrum compared to the already enhanced VCD of fibrils formed from Q repeats 30 and below (Q ? 30).