Expression, purification, immunogenicity and protective efficacy of a recombinant nucleoside hydrolase from Leishmania donovani, a vaccine candidate for preventing cutaneous leishmaniasis

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• 作者：C. Patrick McAtee^a ; ^b ; Christopher A. Seid^a ; ^b ; Molly Hammond^a ; ^b ; Elissa Hudspeth^a ; ^b ; Brian P. Keegan^a ; ^b ; Zhuyun Liu^a ; ^b ; Junfei Wei^a ; ^b ; Bin Zhan^a ; ^b ; Raul Arjona-Sabido^c ; Vladimir Cruz-Chan^c ; Eric Dumonteil^c ; Peter J. Hotez^a ; ^b ; ^d ; ^e ; Maria Elena Bottazzi^a ; ^b ; ^{bottazzi@bcm.edu}
• 关键词：Leishmania donovani ; LdNH36 ; Vaccines ; Neglected tropical diseases ; NTDs
• 刊名：Protein Expression and Purification
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：130
• 期：Complete
• 页码：129-136
• 全文大小：1339 K
• 卷排序：130

文摘

A Leishmania donovani nucleoside hydrolase is expressed and purified from E. coli. Process monitoring and yields are determined by mass spectrometry using the “High 3” method. The L. donovani protein was found to be immunogenic and suitable for vaccine studies. The process represents a scalable, lost-cost production method.