Analysis of liposomes by capillary electrophoresis and their use as carrier in electrokinetic chromatography
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- 作者：Gerhard Bilek ; Leopold Kremser ; Dieter Blaas ; Ernst Kenndler
- 关键词：Liposome ; Phospholipids ; Capillary electrochromatography ; Capillary electrophoresis ; Vesicles ; Pseudostationary phase ; Proteins ; Peptides
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2006
- 出版时间：1 September 2006
- 年：2006
- 卷：841
- 期：1-2
- 页码：38-51
- 全文大小：312 K
文摘
This contribution reviews work about liposomes in the context of electrically driven separation methods in the capillary format. The discussion covers four topics. The one broaches the application of liposomes as pseudo-stationary phases or carriers in vesicle or liposome electrokinetic chromatography (EKC) in the way as microemulsions and micelles are used; it includes the chromatographic use of liposomal bilayers as stationary phases attached to the wall for capillary electrochromatography (CEC). The second topic is the characterization and separation of liposomes as analytes by capillary electrophoresis (CE). Then the determination of distribution coefficients and binding constants between liposomes and ligands is discussed, and finally work dealing with peptides and proteins are reviewed with lipid bilayers as constituents of the electrically driven separation system.