Effect of the HIV epidemic on liver cancer in Africa

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• 作者：Richard J Lessells ; Graham S Cooke
• 关键词：TSP-2 ; Schistosoma japonicum ; Variation ; Tegument ; Immune evasion
• 刊名：The Lancet
• 出版年：2008
• 出版时间：3 May 2008-9 May 2008
• 年：2008
• 卷：371
• 期：9623
• 页码：1504
• 全文大小：60 K

文摘

Members of tetraspanin family expressed on the tegument of Schistosoma mansoni have been regarded as potential protective antigens. In this work, we were surprised to identify seven tetraspanin-2 (TSP-2) homologs of the protective antigen Sm-TSP-2 in Schistosoma japonicum and found that the transcription profiles of Sj-tsp-2 subclasses were highly variable in individual adult worms. RT-PCR revealed that Sj-tsp-2 genes were transcribed in cercariae, schistosomula, adult worms, and eggs; however, Western blot analysis indicated that the Sj-TSP-2 proteins were not expressed in eggs. Immunolocalization assays showed that the Sj-TSP-2 proteins localized on the tegument of schistosomula and adult worms, but exposed only on the surface of adult worms. Mice immunized with the recombinant protein of a single TSP-2 subclass showed no protection, while immunized with a mixture of seven recombinant TSP-2 subclasses provided a moderate protection. Those data implicate that the tegument protein Sj-TSP-2 is involved in immune evasion and the high polymorphism of this molecule must affect its potential as a vaccine candidate.