Antioxidant, antibacterial and cytotoxic effects of the phytochemicals of whole Leucas aspera extract

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• 作者：Md Atiar Rahman^a ; ^b ; ^{211560713@stu.ukzn.ac.za} ; Md Saiful Islam^a
• 关键词：Leucas aspera ; Radical scavenging ; Antibacterial ; Cytotoxic ; Probit
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2013
• 出版时间：April, 2013
• 年：2013
• 卷：3
• 期：4
• 页码：273-279
• 全文大小：438 K

文摘

Objective

To investigate the antioxidant, antibacterial and cytotoxic activity of whole Leucas aspera (Labiatae) (L. aspera) alcoholic extract.

Methods

Whole L. aspera powder was extracted by absolute ethanol (99.50 % ). The ethanolic extract was subjected to antioxidant, antibacterial and brine shrimp lethality assay.

Results

The extract showed potent radical scavenging effect (antioxidant) with IC₅₀ value of (99.58¡À1.22) ¦Ìg/mL which was significant (P<0.01) in comparison to ascorbic acid with IC₅₀ value of (1.25¡À0.95) ¦Ìg/mL. In case of antibacterial screening, the extract showed notable antibacterial effect against the tested microbial strains. Significant (P<0.05) zone of inhibitions against Gram positive Bacillus subtilis [(12.00¡À1.32) mm] and Bacillus megaterium [(13.00¡À1.50) mm], Staphylococcus aureus [(8.00¡À0.50) mm] and Gram negative Salmonella typhi [(6.00¡À0.50) mm], Salmonella paratyphi [(8.00¡À1.00) mm], Shigella dysenteriae [(9.00¡À1.32) mm] and Vibrio cholerae [(9.00¡À0.66) mm] was observed. In brine shrimp lethality bioassay, the extract showed the LC₅₀ value as (181.68¡À2.15) ¦Ìg/mL which was statistically significant (P<0.01) compared to positive control vincristine sulfate [LC₅₀=(0.76¡À0.04) ¦Ìg/mL].

Conclusions

The results demonstrate that the ethanolic extract of L. aspera could be used as antibacterial, pesticidal and various pharmacologic actives.