Production of rabbit monoclonal antibodies against mouse embryonic stem cells and identification of pluripotency-associated surface antigens

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• 作者：Zhou Tan^a ; ¹ ; Jiarong Zhang^a ; ¹ ; Zhongyuan Su^a ; Bin Gu^a ; Xinhang Jiang^a ; Jingfeng Luo^a ; Huijiao Ji^a ; Guangchuan Wang^a ; Bo Tao^a ; Xiaoli Zhao^a ; Liangbiao Chen^b ; Guoliang Yu^c ; Weimin Zhu^c ; Ming Zhang^a ; ^{zhangming_ls@zju.edu.cn}
• 关键词：Rabbit monoclonal antibody ; Embryonic stem cells ; Surface antigens
• 刊名：Journal of Immunological Methods
• 出版年：2011
• 出版时间：28 February 2011
• 年：2011
• 卷：365
• 期：1-2
• 页码：149-157
• 全文大小：811 K

文摘

Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of the blastocyst. ES cell surface molecules are important for the identification, labeling, sorting, quality control and functional studies of ES cells. Currently, knowledge of ES surface molecules is limited. To identify new surface molecules, we generated a panel of rabbit monoclonal antibodies (rMabs) against mouse ES (mES) cells. We identified three monoclonal antibodies that interact with molecules on the mES cell surface and found that the expression of their respective antigens decreased upon mES cell differentiation. The antigen of the rMab ZjuESrMab29 was identified as granulocyte macrophage colony-stimulating factor receptor α (GM-CSFR α) by liquid chromatography–tandem mass spectrometry (LC–MS/MS). This study demonstrated that rabbit monoclonal antibody production via whole-cell immunization could be a practical method for the discovery of stem cell surface antigens.