MiR-631/ZAP70: A novel axis in the migration and invasion of prostate cancer cells

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• 作者：Dewang Fu ^{shenzhirui1@163.com" class="auth_mail" title="E-mail the corresponding author} ; Ben Liu ^{liuben1013@163.com" class="auth_mail" title="E-mail the corresponding author} ; Li E. Zang ^{zanglie1013@163.com" class="auth_mail" title="E-mail the corresponding author} ; Huamao Jiang ; ^{lyjianghm@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：MicroRNA-631 ; ZAP70 ; Prostate cancer ; Migration ; Invasion
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2016
• 出版时间：15 January 2016
• 年：2016
• 卷：469
• 期：3
• 页码：345-351
• 全文大小：1182 K

文摘

MicroRNAs (miRNAs) are short, endogenous non-coding RNA molecules involved in cancer initiation and progression. Using transwell migration and invasion assays, we found that miR-631 inhibited the migration and invasion of prostate cancer (PCa) cells. Bioinformatic algorithms indicated the 3′-untranslated region (3′-UTR) of zeta-associated protein 70 (ZAP70) has a putative binding site for miR-631. We found that miR-631 can bind to the 3′-UTR of ZAP70 and decrease its expression. Further studies confirmed that ZAP70 facilitates PCa cell migration and invasion. Interestingly, using gain- and loss-of function experiments, we found that ZAP70 is a major target of miR-631 and largely mediates its activity. In addition, we further discovered that miR-631 was downregulated and ZAP70 was overexpressed in PCa cell lines and PCa tissues. A concordant inverse correlation between miR-631 and ZAP70 was also found in PCa tissues. In all, our study demonstrates that miR-631 decreases PCa cell migration and invasion by dampening ZAP70 expression.