Effects of Myrtus communis extract treatment in bile duct ligated rats

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• 作者：Ali Sen ; PhD^a ; Sevil Ozkan ; MD^b ; Kafiye Recebova ; MSc^c ; Ozge Cevik ; PhD^d ; Feriha Ercan ; PhD^e ; Elif Kervancıoglu Demirci ; MSc^e ; Leyla Bitis ; PhD^a ; Goksel Sener ; PhD^c ; ^{gsener@marmara.edu.tr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Myrtus communis subsp. communis ; Bile ductal ligation ; Hepatic fibrosis ; Oxidative injury
• 刊名：Journal of Surgical Research
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：205
• 期：2
• 页码：359-367
• 全文大小：2523 K

文摘

The aim of our study was to investigate the antifibrotic and antioxidant effects of Myrtus communis subsp. communis (MC) extract against liver injury and fibrosis occurring in rats with biliary obstruction.

Materials and methods

The rats were randomized into four groups (n = 8). Control group (C), MC-administrated group (MC), the bile duct ligation (BDL), and BDL + MC groups. MC was administered at a dose of 50 mg/kg a day orally for 28 days. In blood samples, total bilirubin, direct bilirubin, alanine aminotransferase, aspartate aminotransferase levels, tumor necrosis factor-α, and interleukin-1β measurement were measured. Oxidative injury was examined by measuring luminol and lucigenin chemiluminescence, malondialdehyde and glutathione levels, superoxide dismutase and myeloperoxidase activities. Transforming growth factor-beta and hydroxyproline levels were measured for analyzing fibrosis. The hepatic injury was also analyzed microscopically.

Results

Plasma total bilirubin, direct bilirubin, alanine aminotransferase, aspartate aminotransferase, tumor necrosis factor-α, and interleukin-1β levels were found significantly high in the BDL group, while these values significantly decreased in the BDL group treated with MC. On the other hand, the glutathione and superoxide dismutase values significantly decreased in the BDL group compared to the control group but increased markedly in BDL + MC group compared to the BDL group. Malondialdehyde levels, myeloperoxidase activity, tissue luminol, lucigenin, transforming growth factor-beta, and hydroxyproline levels when compared with the control group increased dramatically in the BDL group and reduced the MC + BDL group.

Conclusions

Our results suggest that MC protects the liver tissues against oxidative damage following BDL via its radical scavenging and antioxidant activities, which appear to involve the inhibition of tissue neutrophil infiltration.