Attenuated allergic inflammatory response in the lungs during lactation

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• 作者：Julieta E. Ochoa-Amaya^a ; ^d ; Larissa P. Marino^b ; Carla N. Tobaruela^a ; Lilian B. Namazu^a ; Atilio S. Calefi^a ; Rafael Margatho^a ; Vagner Gonç ; alves Jr.^a ; Nicolle Queiroz-Hazarbassanov^a ; Marianne O. Klein^a ; Joã ; o Palermo-Neto^a ; Ana P. Ligeiro de Oliveira^c ; Cristina de O. Massoco^a ; Luciano F. Felicio^a ; ^{lfelicio@usp.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Asthma ; Prolactin ; Lactation ; Lung
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：15 April 2016
• 年：2016
• 卷：151
• 期：Complete
• 页码：281-287
• 全文大小：740 K

文摘

To evaluate the influence of lactation on lung immune function during allergic inflammation.

Main methods

Female rats, 60–90 days old, were divided into three groups: no lung allergy virgins (N group), ovalbumin (OVA)-immunized and sensitized virgins (V group), and OVA-immunized and sensitized lactating females (L group). On gestation day (GD) 10, all animals in L group received a subcutaneous injection of 0.1 mg·kg^− 1 OVA plus aluminum hydroxide. On GD17, the L group received a subcutaneous booster injection of 10 μg OVA plus 10 mg aluminum hydroxide. After 7 days, an inhalatory challenge with 1% OVA was given in 15 min sessions for 3 consecutive days. Animals from the V group received the same treatment, meaning both tests and time intervals between OVA treatment and inhalatory challenge were the same as in the L group. Twenty-four hours after the last inhalation session, the animals were euthanized, and the following tests were performed: total and differential bronchoalveolar lavage (BAL) and femoral marrow lavage (FML) leukocyte counts, quantification of tumor necrosis factor α (TNF-α) and interferon γ (IFN-γ) levels in BAL fluid, and quantification of plasma corticosterone and catecholamine levels.

Key findings

The L group presented lower BAL total leukocyte counts and decreases in the number of eosinophils and macrophages compared with the V group. They also expressed higher BAL IFN-γ and lower plasma corticosterone levels. Plasma norepinephrine levels were higher in the L group than in the N and V groups.

Significance

Lactating female rats presented less intense allergic lung inflammation. Our findings suggest that lactation may protect females from asthmatic crises.