Wnt signaling promotes hindgut fate commitment through regulating multi-lineage genes during hESC differentiation

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• 作者：Xiujuan Zhang^a ; ^b ; Ying Chen^a ; Ying Ye^a ; Jianfeng Wang^c ; Hong Wang^a ; Guohong Yuan^a ; Zhe Lin^d ; Yihui Wu^a ; Yan Zhang^a ; Xinhua Lin^a ; ^e ; ^f ; ^{xinhua.lin@ioz.ac.cn} ; ^{xinhua.lin@cchmc.org}
• 关键词：hESC ; human embryonic stem cell ; RNA-seq ; deep-transcriptome sequencing ; ChIP-seq ; chromatin immunoprecipitation and deep sequencing ; IPA ; Ingenuity Pathway Analysis ; DE ; definitive endoderm ; GO ; gene ontology ; RA ; Retinoic Acid ; Hh ; Hedgehog ; DAVID ; the Database for Annotation ; Visualization ; and Integrated Discovery ; 2D ; two-dimensional ; 3D ; three-dimensional ; DETs ; differentially expressed transcripts ; FW ; FGF4  ; +  ; Wnt3a ; FD ; FGF4  ; +  ; Dkk1 ; TSS ; transcription start site
• 刊名：Cellular Signalling
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：29
• 期：Complete
• 页码：12-22
• 全文大小：2033 K
• 卷排序：29

文摘

Transient inhibition of WNT signal leads to defects in hindgut morphogenesis.RNA-seq and ChIP-seq were performed to identify direct and indirect WNT targets.The WNT target genes are composed of multi-lineage genes.The heterogeneity of APLNR+ cells reflected the diverse mechanisms of WNT signal.