A novel real-time reverse transcription-polymerase chain reaction assay with partially double-stranded linear DNA probe for sensitive detection of hepatitis C viral RNA
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- 作者：Tianfu Liua ; b ; Zhenzhou Wanc ; Jia Liub ; Lingyi Zhanga ; Yanheng Zhoud ; Ke Lanb ; Yihong Hub ; yhhu@ips.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Chiyu Zhangb ; zhangcy1999@ips.ac.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：HCV ; Viral load ; Real-time RT-PCR ; Partially double-stranded linear DNA probe
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：236
- 期：Complete
- 页码：132-138
- 全文大小：1276 K
文摘
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A novel real-time RT-PCR assay for HCV RNA detection was developed.
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A partially double-stranded linear DNA probe was used.
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The assay can detect various HCV genotypes.
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The assay has a high sensitivity (78 IU/ml) and a wide linear detection range (1 × 10e2–1 × 10e11 IU/ml).