Association of polyunsaturated fatty acids in breast milk with fatty acid desaturase gene polymorphisms among Chinese lactating mothers

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• 作者：Zhen Ding^a ; Guo-Liang Liu^b ; Xiang Li^a ; Xue-Yan Chen^a ; Yi-Xia Wu^a ; Can-Can Cui^c ; Xi Zhang^c ; Guang Yang^c ; Lin Xie^a ; ^{xielin@jlu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ANCOVA ; analysis of covariance ; ALA ; a-linolenic acid ; AA ; arachidonic acid ; DGLA ; dihomo-γ-linolenic acid ; DTA ; docosatetraenoic acid ; DHA ; docosahexenoic acid ; df ; degrees of freedom ; EPA ; eicosapentaenoic acid ; FADS ; fatty acid desaturase ; LA ; linoleic acid ; LD ; linkage disequilibrium ; NCBI ; National Center for Biotechnology ; PUFAs ; polyunsaturated fatty acids ; SNPs ; single nucleotide polymorphism ; R2 ; squared correlation coefficient ; GLA ; γ-linoleic acid
• 刊名：Prostaglandins, Leukotrienes and Essential Fatty Acids
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：109
• 期：Complete
• 页码：66-71
• 全文大小：412 K

文摘

The fatty acid desaturase (FADS) controls polyunsaturated fatty acid (PUFA) synthesis in human tissues and breast milk.

Design

Evaluate the influence of 10 single nucleotide polymorphisms (SNPs) and various haplotypes in the FADS gene cluster (FADS1, FADS2, FADS3) on PUFA concentration in the breast milk of 209 healthy Chinese women. PUFA concentrations were measured in breast milk using gas chromatography and genotyping was performed using the Sequenom Mass Array system.

Results

A SNP (rs1535) and 2-locus haplotypes (rs3834458-rs1535, rs1535-rs174575) in the FADS2 gene were associated with concentrations of γ-linoleic acid (GLA) and arachidonic acid (AA) in breast milk. Likewise, in the FADS1 gene, a 2-locus constructed haplotype (rs174547-rs174553) also affected GLA and AA concentration (P<0.05 for all). Minor allele carriers of the SNP and haplotypes described above had lower concentrations of GLA and AA. In the FADS2 gene, the 3-locus haplotype rs3834458-rs1535-rs174575, significantly affected concentrations of GLA but not AA. Pairwise comparison showed that individuals major homozygous for the SNP rs1000778 in the FADS3 gene had lower concentrations of ALA and linoleic acid (LA) in their breast milk.

Conclusion

Polymorphisms in the FADS gene cluster influence PUFA concentrations in the breast milk of Chinese Han lactating women.