Improving the biological activity of the antimicrobial peptide anoplin by membrane anchoring through a lipophilic amino acid derivative

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• 作者：Jack C. Slootweg^a ; Timo B. van Schaik^a ; H. (Linda) C. Quarles van Ufford^a ; Eefjan Breukink^b ; Rob M.J. Liskamp^a ; ^c ; Dirk T.S. Rijkers^a ; ^{D.T.S.Rijkers@uu.nl}
• 关键词：Anoplin ; Antimicrobial peptides ; Lipoamino acids ; Lipopeptides ; Membrane interactions ; Peptide pharmaceuticals ; Peptide synthesis
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2013
• 出版时间：1 July, 2013
• 年：2013
• 卷：23
• 期：13
• 页码：3749-3752
• 全文大小：576 K

文摘

The lipophilic amino acid, (S)-2-aminoundecanoic acid, was synthesized and incorporated at a number of specific positions within the peptide sequence of anoplin. These lipophilic anoplin analogs showed to be more active against Escherichia coli and Staphylococcus aureus compared to native anoplin, while the EC₅₀-value of hemolysis was at least one order of magnitude lower than the MIC values. This was in sharp contrast to the N-acylated anoplin derivative, where a gain in activity also led to a complete loss of selectivity. Thus, the incorporation of a lipophilic amino acid residue into anoplin enhanced the antimicrobial activity, while selectivity towards microbial membranes was retained.