Functional characterization of a Mg2+-dependent O-methyltransferase with coumarin as preferred substrate from the liverwort Plagiochasma appendiculatum
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- 作者：Rui-Xue Xu ; Shuai Gao ; Yu Zhao ; Hong-Xiang Lou ; Ai-Xia Cheng ; aixiacheng@sdu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Liverworts ; Plagiochasma appendiculatum ; O-methyltransferase ; Esculetin ; Scopoletin ; Coumarins
- 刊名：Plant Physiology and Biochemistry
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：106
- 期：Complete
- 页码：269-277
- 全文大小：1613 K
文摘
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AMg2+-dependent O-methyltransferase (PaOMT2) was isolated from a liverwort.
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PaOMT2 catalyzed the methylation of esculetin generating scopoletin.
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The first 40 amino acids enhanced the Kcat and did not affect the localization.
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PaOMT2 is located in the cytoplasm.