Two-photon excitation action cross-sections of the autofluorescent proteins

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• 作者：Blab ; Gerhard A. ; Lommerse ; Piet H.M. ; Cognet ; Laurent ; Harms ; Gregory S. ; Schmidt ; Thomas
• 刊名：Chemical Physics Letters
• 出版年：2001
• 出版时间：December 14, 2001
• 年：2001
• 卷：350
• 期：1-2
• 页码：71-77
• 全文大小：123 K

文摘

We report on the values of the two-photon excitation action cross-sections of commercially available enhanced cyan, green, yellow, and red fluorescent proteins. The two-photon absorption spectra are very similar in shape to those measured for one-photon absorption. However, they exhibit a significant blueshift, which is attributed to the participation of a vibrational mode in the two-photon absorption process. The two-photon spectra are compared to that of flavine mononucleotide, which constitutes the main source of autofluorescence in mammalian cells. The definition of a relative detection yield between the autofluorescent proteins and flavine allows us to quantify the applicability of autofluorescent proteins in two-photon single-molecule studies in living cells.