Decorating outer membrane vesicles with organophosphorus hydrolase and cellulose binding domain for organophosphate pesticide degradation
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- 作者：Fu-Hsiang Su ; Ian Dominic Flormata Tabañ ; ag ; Chih-Yun Wu ; Shen-Long Tsai ; stsai@mail.ntust.edu.tw" class="auth_mail" title="E-mail the corresponding author
- 关键词：Outer membrane vesicles ; Organophosphorus hydrolase ; Cellulose binding module ; Paraoxon ; Biodegradation
- 刊名：Chemical Engineering Journal
- 出版年：2017
- 出版时间：15 January 2017
- 年：2017
- 卷：308
- 期：Complete
- 页码：1-7
- 全文大小：1394 K
文摘
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An OPH and a CBD were appended to OMVs via genetic fusion.
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The engineered OMVs exhibited an enhanced activity toward paraoxon degradation.
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Recovery of the engineered OMVs could be achieved easily via cellulose pull-down.
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OPHs tethered on OMVs revealed improved temperature, pH and long-term stabilities.
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