Polyamine oxidase activity in lymphoid tissues of glucocorticoid-treated rats
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- 作者：Ferioli ; Maria Elena ; Pinotti ; Orietta ; Pirona ; Lorenza
- 关键词：polyamine oxidase ; spermidine/spermine N1-acetyltransferase ; enzymes of the catabolic pathway of polyamines ; glucocorticoids ; effect on polyamine catabolism ; thymus ; spleen ; rat
- 刊名：Biochemical Pharmacology
- 出版年：1999
- 出版时间：December 15, 1999
- 年：1999
- 卷：58
- 期：12
- 页码：1907-1914
- 全文大小：624 K
文摘
Glucocorticoids are known to negatively affect lymphoid tissues, in which they cause programmed cell death. Polyamine depletion, which occurs in glucocorticoid-treated animals by inhibition of biosynthesis and induction of acetylation, may represent a signal to thymocytes for progression into the apoptotic program. Since catalysis of polyamines by the catabolic pathway produces hydrogen peroxide as a by-product, it has been suggested that the apoptotic process may be, in part, due to oxidative stress as a result of hydrogen peroxide production. In order to verify whether polyamine oxidase (EC 1.5.3.11) may play a role in the process, we examined the activity of the enzyme in the thymus and spleen of glucorticoid-treated rats. We administered dexamethasone (4 mg/kg) or two different doses of corticosterone (4 mg/kg or 30 mg/kg) to rats, which were killed 8 or 24 hr after hormone injection. We found that corticosterone and dexamethasone affected polyamine oxidase activity in both tissues, with an opposite dose-dependent effect of the natural hormone in the thymus. The decrease and increase in polyamine oxidase after the two doses of corticosterone were correlated with the absence and the occurrence of DNA fragmentation, respectively. Moreover, corticosterone affected polyamine oxidase activity earlier (8 hr) than dexamethasone (24 hr), but the synthetic hormone was more efficient than the natural hormone in thymic polyamine depletion. The polyamine oxidase response may represent an important event in lymphoid tissues after glucocorticoid treatment, suggesting a role of the enzyme in the catabolic effects exerted by the two hormones.