High-Throughput Sequencing Reveals Immunological Characteristics of the TRB-/IgH-CDR3 Region of Umbilical Cord Blood

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• 作者：Changlong Guo ; MD ; PhD¹ ; ^&lowast ; ; Qidi Wang ; MD ; PhD¹ ; ^&lowast ; ; Xiaofang Cao ; MD¹ ; ^{mybrother1984@163.com" class="auth_mail" title="E-mail the corresponding author} ; Ying Yang ; MD ; PhD¹ ; Xin Liu ; PhD¹ ; ² ; Lisha An ; MD¹ ; Ruikun Cai ; PhD¹ ; Meng Du ; MD¹ ; ³ ; Guangyu Wang ; MD¹ ; Yue Qiu ; MD¹ ; ³ ; Zuoqi Peng ; MD¹ ; Jian Han ; MD ; PhD⁴ ; Shuhua Ni ; PhD⁵ ; Xuerui Tan ; MD ; PhD⁵ ; Li Jin ; MD⁶ ; Song Yu ; MD⁷ ; Huiying Wang ; MD⁸ ; Chunlin Wang ; PhD¹ ; ⁹ ; Xingyu Wang ; MD ; PhD¹ ; ² ; Xu Ma ; MD ; PhD¹ ; ³
• 关键词：umbilical cord blood ; high-throughput sequencing ; TRB-/IgH-CDR3 repertoires
• 刊名：The Journal of Pediatrics
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：176
• 期：Complete
• 页码：69-78.e1
• 全文大小：2565 K

文摘

To compare the differences of immunological characteristics between newborn and adults, we performed high-throughput sequencing to reveal the diversity of umbilical cord blood and adult peripheral blood at both T-cell receptor beta chain (TRB) and immunoglobulin heavy chain (IGH) levels.

Study design

High-throughput sequencing was performed to analyze the expression of TRB-CDR3 and IGH-CDR3 in circulating T and B cells isolated from 20 healthy adults, 56 pregnant women, and 40 newborns.

Results

Our results revealed different immunological characteristics between newborn and adults, such as distinctive complementarity determining region 3 (CDR3) lengths, usage bias of variable and joining segments, random nucleotide addition, a large number of unique CDR3 peptides, and a greater repertoire diversity. Moreover, each newborn had a distinctive TRB-/IGH-CDR3 repertoire that was independent of the maternal immune status.

Conclusions

This study presents comprehensive, unrestricted profiles of the TRB/IGH-CDR3 repertoire of newborns, pregnant women, and healthy adults at a sequence-level resolution. Our data may contribute to a better understanding of the immune system of newborns and benefit the efficient application of umbilical cord blood transplantation in future.