Increase of segmental mobility through insertion of a flexible linker in split point of firefly luciferase

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• 作者：Parisa Bahmani ; Saman Hosseinkhani ; ^{saman_h@modares.ac.ir}
• 关键词：FL1 ; mutant with one flexible linker ; FL2 ; mutant with two flexible linkers ; ANS ; 8-anilino-1-naphthalenesulfonic acid ; PMSF ; phenylmethanesulfonyl fluoride
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：94
• 期：part_PB
• 页码：762-770
• 全文大小：1212 K
• 卷排序：94

文摘

The crystal structure of Photinus pyralis luciferase shows a unique molecular architecture consisting of a large N-terminal domain and a small C-terminal domain which is separated by a wide cleft. Protein engineering methods attempts to design the peptide linkers that make a connection between different protein domains or subunits to allow for separating domains and improve kinetics and structural features of proteins. In regard to this; introduction of a flexible linker at split point of luciferase which has a strong self-association activity, may leads to conformational change and improve general flexibility of protein. In this study, two flexible linkers in the split point of luciferase are introduced in order to test the effect of linker on flexibility of luciferase activity. Glycine-rich linkers are introduced into P. pyralis firefly luciferase to make two separate mutant enzymes. Enzymatic properties of mutant and native forms were measured using luminescence assay. Results show that lengthening of luciferase domains through insertion of a flexible linker did not affect bioluminescence emission spectra. Also adding linkers do not have remarkable effect on thermostability. The Km values of mutants were increased compared to native form, indicating lower affinity of mutants toward substrates.