Identification of amino acid residues responsible for high initial luminescence intensity in a calcium-binding photoprotein, clytin-II
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- 作者：Satoshi Inouye ; sinouye@jnc-corp.co.jp" class="auth_mail" title="E-mail the corresponding author ; Yuiko Sahara-Miura
- 关键词：Photoprotein ; Chimeric protein ; Aequorin ; GPCR reporter assay ; Mitochondrial signal peptide
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：8 January 2016
- 年：2016
- 卷：469
- 期：2
- 页码：300-305
- 全文大小：833 K
文摘
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Clytin-II (CL-II) is a Ca2+-binding photoprotein from the jellyfish Clytia gregaria.
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CL-II is an isotype of CL-I with 23 different amino acids in 189 amino acid residues.
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CL-II shows the highest initial luminescence intensity among photoproteins.
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Amino acids responsible for high initial luminescence intensity were determined.
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CL-I mutant substituted with four amino acids shows the similar properties to CL-II.