A novel recombinant Meq protein based dot-ELISA for rapid and confirmatory diagnosis of Marek’s disease induced lymphoma in poultry
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- 作者：M. Asok Kumara ; drasokvet@gmail.com" class="auth_mail" title="E-mail the corresponding author ; R. Barathidasana ; barathdassss@gmail.com" class="auth_mail" title="E-mail the corresponding author ; M. Palanivelua ; drpalvet@gmail.com" class="auth_mail" title="E-mail the corresponding author ; S.D. Singha ; sdsingh2005@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Mohd Yaqoob Wanib ; mywani27@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Yashpal Singh Malikc ; malikyps@ivri.res.in" class="auth_mail" title="E-mail the corresponding author ; R. Singhd ; rajendra_singh5747@rediffmail.com" class="auth_mail" title="E-mail the corresponding author ; K. Dhamaa ; kdhama@rediffmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：MEQ ; MDV EcoRI-Q ; HIS ; Hyper Immune Serum ; LB ; Luria Broth ; ELISA ; Enzyme-Linked Immunosorbent Assay ; PBS(T) ; phosphate buffered saline (tween 20) ; RIPA ; buffer-radioimmunoprecipitation assay buffer ; BSA ; Bovine serum albumen ; RT ; room temperature ; NCM ; Nitrocellulose membrane ; IBH ; Inclusion Body hepatitis ; PO ; peroxidase ; ALKP ; alkaline phosphatase ; LL ; lymphoid leucosis ; RE ; reticuloendotheliosis ; IBH ; Inclusion Body hepatitis
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：236
- 期：Complete
- 页码：271-280
- 全文大小：1899 K
文摘
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A specific, rapid and cost-effective technique of dot-ELISA was developed for detecting Meq protein in tissues of Marek’s disease virus (MDV) infected birds.
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The dot-ELISA diagnosed MDV induced lymphoma in poultry with better sensitivity and high specificity than conventional PCR.
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This dot-ELISA could also be helpful in differentiating other tumor causing viruses in poultry.