CRISPR interference-guided balancing of a biosynthetic mevalonate pathway increases terpenoid production
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- 作者：Seong Keun Kima ; b ; Gui Hwan Hana ; Wonjae Seonga ; b ; Haseong Kima ; b ; Seon-Won Kimc ; Dae-Hee Leea ; b ; dhlee@kribb.re.kr" class="auth_mail" title="E-mail the corresponding author ; Seung-Goo Leea ; b ; sglee@kribb.re.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：CRISPR ; Clustered regularly interspaced short palindromic repeats ; CRISPRi ; Clustered regularly interspaced short palindromic repeats interference ; MVA ; mevalonate ; sgRNA ; single guide RNA ; dCas9 ; inactive Cas9 ; IPP ; isopentenyl diphosphate ; DMAPP ; dimethylallyl diphosphate ; MEP ; methylerythritol 4-phosphate ; DXP ; 1-deoxy-D-xylulose 5-phosphate ; HMG-CoA ; 3-hydroxy-3-methyl-glutaryl-CoA ; LB ; Luria-Bertani ; TB ; terrific broth ; PCR ; polymerase chain reaction ; UTR ; untranslated region ; T7RNAP ; T7 RN
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：38
- 期：Complete
- 页码：228-240
- 全文大小：957 K
文摘
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A regulatable CRISPR interference (CRISPRi) system was developed.
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Developed CRISPRi system was successfully applied to terpenoid production.
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CRISPRi-guided balancing of MVA pathway greatly enhanced terpenoid production.
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Regulatable CRISPRi system can be a powerful tool for metabolic engineering.