Increased expression of high but not low molecular weight heat shock proteins in resectable lung carcinoma

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• 作者：Michils ; Alain ; Redivo ; Myriam ; Zegers de Beyl ; Valé ; rie ; de Maertelaer ; Viviane ; Jacobovitz ; Danielle ; et. al.
• 关键词：Lung cancer ; Heat shock proteins ; Ubiquitin ; HSP-27 ; HSP-60 ; HSP-70
• 刊名：Lung Cancer
• 出版年：2001
• 出版时间：July, 2001
• 年：2001
• 卷：33
• 期：1
• 页码：59-67
• 全文大小：118 K

文摘

Strong expression of high-molecular-weight (HMW) heat-shock proteins (HSP) by lung carcinoma has been documented using immunohistochemistry. Far less is known about the expression of low-molecular-weight (LMW) HSP in lung cancer. We compared the quantitative expression of HMW (HSP-60, HSP-70) and LMW (HSP-27, ubiquitin) HSP in tumor and non-tumor lung tissue obtained from 47 patients undergoing surgical resection of lung carcinoma. HSP levels were determined in cell lysates from tissue samples by ELISA using streptavidin-biotin technology. Results were normalized to total protein content measured by spectrophotometry. Compared to disease-free lung tissue, tumor tissue samples showed higher levels of both HSP-60 (median value: 227 pg versus 96 pg per mg protein (P<0.001 by Wilcoxon Rank test for paired data) and HSP-70 (median value: 525 ng versus 401 ng per mg protein (P