Recommended protocol for the Syrian hamster embryo (SHE) cell transformation assay

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• 作者：Marie-Aline Maire^a ; ^{marie-aline.maire@univ-metz.fr} ; Kamala Pant^b ; Pascal Phrakonkham^c ; Albrecht Poth^d ; Karl-Rainer Schwind^e ; Claudine Rast^a ; Shannon Wilson Bruce^b ; Jamie E. Sly^b ; Susanne Bohnenberger^d ; Thorsten Kunkelmann^d ; Markus Schulz^e ; Paule Vasseur^a
• 关键词：CMF-HBSS ; calcium- and magnesium-free Hank's balanced solution ; CMF-PBS ; calcium- and magnesium-free phosphate buffered saline ; CTA ; cell transformation assay ; DMEM ; Dulbecco's Modified Eagle's Medium ; DRF ; dose range finding ; ECVAM ; European Centre for t
• 刊名：Mutation Research/Genetic Toxicology and Environmental Mutagenesis
• 出版年：2012
• 出版时间：11 April, 2012
• 年：2012
• 卷：744
• 期：1
• 页码：76-81
• 全文大小：257 K

文摘

The Syrian hamster embryo (SHE) cell transformation assay (CTA) is a short-term in vitro assay recommended as an alternative method for testing the carcinogenic potential of chemicals. SHE cells are ¡°normal?cells since they are diploid, genetically stable, non-tumourigenic, and have metabolic capabilities for the activation of some classes of carcinogens. The CTA, first developed in the 1960s by Berwald and Sachs (1963,1964) , is based on the change of the phenotypic feature of cell colonies expressing the first steps of the conversion of normal to neoplastic-like cells with oncogenic properties. Pienta et al. (1977) developed a protocol using cryopreserved cells to enhance practicality of the assay and limit sources of variability. Several variants of the assay are currently in use, which mainly differ by the pH at which the assay is performed. We present here the common version of the SHE pH 6.7 CTA and SHE pH 7.0 CTA protocols used in the ECVAM (European Centre for the Validation of Alternative Methods) prevalidation study on CTA reported in this issue. It is recommended that this protocol, in combination with the photo catalogues presented in this issue, should be used in the future and serve as a basis for the development of the OECD test guideline.