Synthesis of biotinylated sialoside to probe CD22–ligand interactions
详细信息 查看全文
- 作者：Hajjaj H.M. Abdu-Allah ; Kozo Watanabe ; Koji Hayashizaki ; Yuki Iwayama ; Hiromu Takematsu ; Yasunori Kozutsumi ; Takeshi Tsubata ; Hideharu Ishida ; Makoto Kiso
- 关键词：Biotinylated CD22 ligand ; Hanessian reaction ; Click chemistry
- 刊名：Tetrahedron Letters
- 出版年：2009
- 出版时间：5 August 2009
- 年：2009
- 卷：50
- 期：31
- 页码：4488-4491
- 全文大小：397 K
文摘
Biotin conjugate of the most potent ligand for mouse CD22 was designed and synthesized. The key synthetic step involved the dual capability of Hanessian reaction for debenzylation and simultaneous conversion of the anomeric p-methoxyphenyl group into the corresponding phenylthioglycoside in one step. Competition enzyme-linked immunosorbent assay (ELISA) for testing the binding affinity of synthetic sialosides was developed based on this biotinylated ligand.