Development of a simplified purification method for a novel formaldehyde dismutase variant from Pseudomonas putida J3

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• 作者：Lisa Blaschke^a ; Wenke Wagner^b ; Christina Werkmeister^b ; Marion Wild^b ; Adrian Gihring^b ; Steffen Rupp^a ; ^b ; Susanne Zibek^a ; ^b ; ^{susanne.zibek@igb.fraunhofer.de}
• 关键词：ADH ; alcohol dehydrogenase ; AOX ; alcohol oxidase ; CV ; column volume ; FDH ; formate dehydrogenase ; FDM ; formaldehyde dismutase ; IMAC ; immobilized metal affinity chromatography ; MBP ; maltose binding protein ; MWCO ; molecular weight cut-off
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：10 January 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：69-75
• 全文大小：898 K
• 卷排序：241

文摘

A novel formaldehyde dismutase gene was identified in Pseudomonas putida J3. High expression levels were obtained in an Escherichia coli rhamnose-inducible system (51 U mg−1). Chaperone GroESL coexpression increased yield of activity in raw extracts 8.6 fold. First report of a functional formaldehyde dismutase with a C-terminal 6xHis-Tag allowing simple affinity purification. Formaldehyde dismutase will be useful for biomethanol production from biogas.