Structural Dynamics in Ras and Related Proteins upon Nucleotide Switching
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- 作者：Rane A. Harrison1 ; Jia Lu2 ; Martin Carrasco2 ; John Hunter2 ; Anuj Manandhar2 ; Sudershan Gondi2 ; Kenneth D. Westover2 ; kenneth.westover@utsouthwestern.edu" class="auth_mail" title="E-mail the corresponding author ; John R. Engen1 ; j.engen@northeastern.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：MD ; molecular dynamics ; HDX ; hydrogen&ndash ; deuterium exchange ; MS ; mass spectrometry ; P-loop ; phosphate-binding loop ; GDP ; guanosine diphosphate ; GTP ; guanosine triphosphate ; GAP ; GTPase-activating protein ; SIIP ; switch II pocket ; GMPPNP ; guanylyl imidodiphosphate ; RMSF ; root mean square fluctuation
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：20 November 2016
- 年：2016
- 卷：428
- 期：23
- 页码：4723-4735
- 全文大小：2122 K
文摘
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HDX MS and molecular dynamics simulations of K-Ras, H-Ras, N-Ras, R-Ras, Rap1B, CDC42, Rac1, and RhoA
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Ras subfamily member HDX MS is distinct from Rho subfamily member HDX MS.
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Dynamics and primary/tertiary structure conservation do not correlate.
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We identified a subfamily-dependent correlation between switch II and P-loop.